WC 1M Post Challenge - You Ready?!

[hope4wp]

re: WC 100K Post Challenge - You Ready?!

shame

I might punch you because i call you bhai

 

[hope4wp]

re: WC 100K Post Challenge - You Ready?!

I wonder if he knows in history that many of the rulers used to have many wives and many concubines. I wonder what he thought they did with them...

Distribute them to the kingdom! =))))))) lol

 

[hope4wp]

re: WC 100K Post Challenge - You Ready?!

Hey Shu i installed that app! and i suck

 

[SlingLaker9811]

re: WC 100K Post Challenge - You Ready?!

It's for long term storage.

When they are in a liquid culture you have to work immediately with them, for some applications you can them keep on ice for a few hours, but that's about it. On agar plates you can keep them up to a month, depending on the strain and the antibiotic resistance. However if you want to store them for months or years, you keep them in a freezer at -80?C.

You can then just take a tooth-pick, scratch a little bit off the frozen surface and streak it out on another agar plate, and they'll happily grow again.

You can do something similar with cell cultures, but that's much more complex and you freeze them in liquid nitrogen or in the gaseous phase of liquid nitrogen.

Microbiology is so interesting! I'm gonna go into clinical research after my Mbbs. Maybe genetics/Molecular/cell biology as specialisation? Hmm..... :Thinking:

 

[SlingLaker9811]

re: WC 100K Post Challenge - You Ready?!

It's for long term storage.

When they are in a liquid culture you have to work immediately with them, for some applications you can them keep on ice for a few hours, but that's about it. On agar plates you can keep them up to a month, depending on the strain and the antibiotic resistance. However if you want to store them for months or years, you keep them in a freezer at -80?C.

You can then just take a tooth-pick, scratch a little bit off the frozen surface and streak it out on another agar plate, and they'll happily grow again.

You can do something similar with cell cultures, but that's much more complex and you freeze them in liquid nitrogen or in the gaseous phase of liquid nitrogen.

Have you also inserted the antibiotic gene for ampicillin and tetracycline in the bacteria in one of your experiments? If so what happened? Do the bacteria really change colour?

 

[anon(7900571)]

re: WC 100K Post Challenge - You Ready?!

It's kind of a weird feeling though. We're getting the first major update to the OS with the first major firmware update and we're losing capabilities because the phones are first gen. How sad. I sort of guessed this would happened but didn't think it would be so soon.

Lol apple did the same with my old iPad. If you have used ios7 you know the new UI elements , transparency/glossy effects. Looks eye candy. So when the first beta was made available I tried on my iPad 3. It was smooth and I was in love with those eye candy effects with beta 5 apple disabled them -_-. I thought it was a bug . No it was in gold version as well as final version. NC is plain dull black colour with CC is grey(puke). When first jailbreak was available. I downloaded some tweaks from cydia and they brought those effects back with a surprise. Air-Drop which apple said isn't compatible with my iPad. It was smooth and there was no impact on battery life. Wonder why apple disabled them. I believe that's because to boost up the sales of newer ipad and to set a difference btw older ipad and newer iPad. Iphone4s got all those effects and my iPad didn't (they have the same specs , iPhone 4s has 512MB ram and A5 processor where as iPad 3 has A5X processor Where X-=Quad core processor ,and 1Gb ram ) Shame on you apple. From that I am on a commitment I wont purchase any apple products -_-

 

[anon(7900571)]

re: WC 100K Post Challenge - You Ready?!

I might punch you because i call you bhai

you may , I wont say No because you are my sister

 

[anon(7900571)]

re: WC 100K Post Challenge - You Ready?!

Hey Shu i installed that app! and i suck

lol keep on trying. which section/category are playing ? There are many more , just go through all the sections and I am sure you might find your fav

 

[SlingLaker9811]

re: WC 100K Post Challenge - You Ready?!

Lol apple did the same with my old iPad. If you have used ios7 you know the new UI elements , transparency/glossy effects. Looks eye candy. So when the first beta was made available I tried on my iPad 3. It was smooth and I was in love with those eye candy effects with beta 5 apple disabled them -_-. I thought it was a bug . No it was in gold version as well as final version. NC is plain dull black colour with CC is grey(puke). When first jailbreak was available. I downloaded some tweaks from cydia and they brought those effects back with a surprise. Air-Drop which apple said isn't compatible with my iPad. It was smooth and there was no impact on battery life. Wonder why apple disabled them. I believe that's because to boost up the sales of newer ipad and to set a difference btw older ipad and newer iPad. Iphone4s got all those effects and my iPad didn't (they have the same specs , iPhone 4s has 512MB ram and A5 processor where as iPad 3 has A5X processor Where X-=Quad core processor ,and 1Gb ram ) Shame on you apple. From that I am on a commitment I wont purchase any apple products -_-

Wish Surface Pro 3 was available in India, no?

 

[anon(7900571)]

re: WC 100K Post Challenge - You Ready?!

Wish �� Surface Pro 3 was available in India, no? ��

I am still waiting for first surface 1 to be released here , then I will think about surface 2 , surface pro 3 Just kidding lol you have many alternatives here :wink:

 

[N_LaRUE]

re: WC 100K Post Challenge - You Ready?!

Lol apple did the same with my old iPad. If you have used ios7 you know the new UI elements , transparency/glossy effects. Looks eye candy. So when the first beta was made available I tried on my iPad 3. It was smooth and I was in love with those eye candy effects with beta 5 apple disabled them -_-. I thought it was a bug . No it was in gold version as well as final version. NC is plain dull black colour with CC is grey(puke). When first jailbreak was available. I downloaded some tweaks from cydia and they brought those effects back with a surprise. Air-Drop which apple said isn't compatible with my iPad. It was smooth and there was no impact on battery life. Wonder why apple disabled them. I believe that's because to boost up the sales of newer ipad and to set a difference btw older ipad and newer iPad. Iphone4s got all those effects and my iPad didn't (they have the same specs , iPhone 4s has 512MB ram and A5 processor where as iPad 3 has A5X processor Where X-=Quad core processor ,and 1Gb ram ) Shame on you apple. From that I am on a commitment I wont purchase any apple products -_-

Apple is known for doing things like this to promote their newer products. They did this with several of their devices.

I'm just surprised that Nokia seems to be following the same path. Well not surprised, Nokia doesn't have the greatest track record for updates and they really screwed over some countries when it came to updates in the past. But I thought things would be different. I just hope that I will get an update on my device due to it being locked to a carrier. :/ It's been such a long wait.

 

[N_LaRUE]

re: WC 100K Post Challenge - You Ready?!

I am still waiting for first surface 1 to be released here , then I will think about surface 2 , surface pro 3 Just kidding lol you have many alternatives here :wink:

If you decide to buy the Surface get it from the US. The EU is too expensive.

 

[anon(7900571)]

re: WC 100K Post Challenge - You Ready?!

If you decide to buy the Surface get it from the US. The EU is too expensive.

I ended up buying a new notebook. Lenovo flex 10 quad core mode :smile: I use it for indexing forums, creating presentation and some WPC work :wink:

 

[N_LaRUE]

re: WC 100K Post Challenge - You Ready?!

I ended up buying a new notebook. Lenovo flex 10 quad core mode :smile: I use it for indexing forums, creating presentation and some WPC work :wink:

Nice. I have to wait a bit to get a new laptop. I'm seriously considering re-installing the OS. My laptop has some issues. I really hate re staging. :/

 

[anon(7900571)]

re: WC 100K Post Challenge - You Ready?!

Nice. I have to wait a bit to get a new laptop. I'm seriously considering re-installing the OS. My laptop has some issues. I really hate re staging. :/

that sucks , try clearing temp files using disk clean-up , check your hard drive if is it fragmented ?

 

[N_LaRUE]

re: WC 100K Post Challenge - You Ready?!

that sucks , try clearing temp files using disk clean-up , check your hard drive if is it fragmented ?

No it's to do with the WiFi adapter and some other things. It's been running really slow lately, which it shouldn't be so I think a fresh start is best.

 

[anon(7900571)]

re: WC 100K Post Challenge - You Ready?!

No it's to do with the WiFi adapter and some other things. It's been running really slow lately, which it shouldn't be so I think a fresh start is best.

don't forget to backup all your important data Rue

 

[N_LaRUE]

re: WC 100K Post Challenge - You Ready?!

don't forget to backup all your important data Rue

LOL! Of course. I have a 2 TB NAS. I always backup my important stuff.

 

[Chregu]

re: WC 100K Post Challenge - You Ready?!

Have you also inserted the antibiotic gene for ampicillin and tetracycline in the bacteria in one of your experiments? If so what happened? Do the bacteria really change colour?  

Antibiotic resistances are a main tool of bacteriology. It has many applications, mostly for selection purposes.

If you bring a plasmid (a DNA fragment that works like a little genome) into bacteria, you usually have a antibiotic cassette on it. The bacteria that take up the plasmid will express proteins that make them resistant to the antibiotics and will thus survive and/or grow, while bacteria without them will die or not grow. After bacteria have taken up the plasmid and are selected for it, you have to keep growing them in the antibiotics, as they would lose the plasmid after a while without it (replicating the plasmid costs time and energy, if they don't need it it will get lost as the bacteria without it will just grow faster).

Another application is the selection for successful transductants after bringing a gene into the bacteria with viruses.

It can also be used as a negative selection tool, to select for the bacteria that have lost the sensibility to antibiotics (you have to screen for them on plates with the antibiotics and without them).

This is only a selection of the most broadly used applications of antibiotics. It's an elementary tool of our work.

Now about the color changing part. There are multiple different screening methods that rely on colonies that change color. Bacteria do however not just change color when they express a resistance gene. We use multiple antibiotics (I work with ampicillin and tetracycline on a daily basis) and they don't change color only because of the resistance cassette.

I don't know to what screen or gene you were referring to

 

[hope4wp]

re: WC 100K Post Challenge - You Ready?!

lol keep on trying. which section/category are playing ? There are many more , just go through all the sections and I am sure you might find your fav

HIMYM that was the only one i was good in