WC 1M Post Challenge - You Ready?!
- Thread starter BIGPADDY
- Start date
MSFTisMIA
New member
re: WC 100K Post Challenge - You Ready?!
I wouldn't tie him to a steak because I don't want to disrespect the meat, but to a floating piece of wood may not be a bad idea...
...
We have Sahib who uses an iPod. We have MIA who uses Android. I use both WP and Android. There are others using iPhones and Android devices. I don't think we'll get out the burning pitch and tie you to a steak just yet.
I wouldn't tie him to a steak because I don't want to disrespect the meat, but to a floating piece of wood may not be a bad idea...
...
N_LaRUE
New member
re: WC 100K Post Challenge - You Ready?!
LOL! Well a little charged Chregu never hurt a steak I'm sure.
LOL! Well a little charged Chregu never hurt a steak I'm sure.
sahib lopez
New member
re: WC 100K Post Challenge - You Ready?!
yeah yeah but still can't blame me for being excited
Sent from my iPod touch using Tapatalk
Relax, go there without too many expectations. People change in 4 years, just try to have a good time and be open for everything
yeah yeah but still can't blame me for being excited Sent from my iPod touch using Tapatalk
sahib lopez
New member
WPC 75K Post Challenge - You Ready?!
Burn the infidel! *hides my iPod *
Sent from my iPod touch using Tapatalk
LOL! Well a little charged Chregu never hurt a steak I'm sure.
Burn the infidel! *hides my iPod *
Sent from my iPod touch using Tapatalk
MSFTisMIA
New member
re: WC 100K Post Challenge - You Ready?!
You know, I was really thinking about getting Nexus 5 and this MSFT Android talk may spur those efforts. I'm honestly so so about it, but I may try to go Google lite if I do so - no Gmail and some other things. I remember those pure AOSP/Gapp free ROM days...but that **** is done now...
You know, I was really thinking about getting Nexus 5 and this MSFT Android talk may spur those efforts. I'm honestly so so about it, but I may try to go Google lite if I do so - no Gmail and some other things. I remember those pure AOSP/Gapp free ROM days...but that **** is done now...
sahib lopez
New member
re: WC 100K Post Challenge - You Ready?!
Sad to see msft is thinking about an android device . No matter how much people say it's the way to go, it's not my go to device . I use my devices as an extension to my tablet not the other they way around. Msft these days baffles me with the choices they are making
Sent from my iPod touch using Tapatalk
Sad to see msft is thinking about an android device . No matter how much people say it's the way to go, it's not my go to device . I use my devices as an extension to my tablet not the other they way around. Msft these days baffles me with the choices they are making
Sent from my iPod touch using Tapatalk
sd173
New member
re: WC 100K Post Challenge - You Ready?!
Is it not possible to play a video through an SD card on WP 8.1? That's what it seems like and that would be really dumb if it's true.
Is it not possible to play a video through an SD card on WP 8.1? That's what it seems like and that would be really dumb if it's true.
N_LaRUE
New member
re: WC 100K Post Challenge - You Ready?!
Have you tried a different video? I was thinking that maybe the other video had DRM or something. But then it wouldn't surprise me if MS didn't allow video playback off SD card but then I haven't seen complaints about it either.
Have you tried a different video? I was thinking that maybe the other video had DRM or something. But then it wouldn't surprise me if MS didn't allow video playback off SD card but then I haven't seen complaints about it either.
sd173
New member
re: WC 100K Post Challenge - You Ready?!
I only have one Xbox video file but I'm thinking it has to be something with the DRM since the Windows 8.1 file explorer dialogue box said that the file would lose some permissions if I moved it to the SD card.
I only have one Xbox video file but I'm thinking it has to be something with the DRM since the Windows 8.1 file explorer dialogue box said that the file would lose some permissions if I moved it to the SD card.
SlingLaker9811
Banned
sd173
New member
re: WC 100K Post Challenge - You Ready?!
The worst part is I can't move the same video that I downloaded through the WP 8.1 app since it's stuck in the app and not visible though the Files app.
The worst part is I can't move the same video that I downloaded through the WP 8.1 app since it's stuck in the app and not visible though the Files app.
Chregu
New member
re: WC 100K Post Challenge - You Ready?!
I'm trying to create a bacteria strain that is very important for my experiments. It's already pretty sick and I need to knock-out an additional gene. It didn't work. It was the second time I tried...
I'm trying to create a bacteria strain that is very important for my experiments. It's already pretty sick and I need to knock-out an additional gene. It didn't work. It was the second time I tried...
Chregu
New member
re: WC 100K Post Challenge - You Ready?!
I have to try out the LG G3 in a store. I think it's an amazing phone that is much cheaper than the S5 or M8. But unfortunately it's also huge.
I have to try out the LG G3 in a store. I think it's an amazing phone that is much cheaper than the S5 or M8. But unfortunately it's also huge.
SlingLaker9811
Banned
re: WC 100K Post Challenge - You Ready?!
How do you create it(the strain)? Do you have to insert the desired gene into a vector and then it binds with the plasmid of the bacteria to produce the desired effect?
How do you create it(the strain)? Do you have to insert the desired gene into a vector and then it binds with the plasmid of the bacteria to produce the desired effect?
Chregu
New member
re: WC 100K Post Challenge - You Ready?!
It's a double recombination with a suicide vector. To knock-out the gene first around 600 bp of the DNA upstream and downstream of the gene is cloned into a vector. After bringing the vector into the bacteria, these two regions then recombine with a very low efficiency and remove the gene while bringing in a antibiotic resistance cassette and a sacB, a special gene that is important later. By selection for the antibiotic resistance colonies with the recombination can by gained. These will then be grown in a liquid culture again to make a second recombination, losing the resistance cassette and sacB. You then do a second selection by growing the bacteria on sucrose. Sucrose kills all bacteria that have the sacB gene.
The now yielded colonies have to be screened for lost antibiotic resistance (to avoid choosing bacteria with a mutation on sacB) and then a colony PCR is performed to confirm the knock-out.
Everything worked until the last step. But no colony had lost the gene...
It's a double recombination with a suicide vector. To knock-out the gene first around 600 bp of the DNA upstream and downstream of the gene is cloned into a vector. After bringing the vector into the bacteria, these two regions then recombine with a very low efficiency and remove the gene while bringing in a antibiotic resistance cassette and a sacB, a special gene that is important later. By selection for the antibiotic resistance colonies with the recombination can by gained. These will then be grown in a liquid culture again to make a second recombination, losing the resistance cassette and sacB. You then do a second selection by growing the bacteria on sucrose. Sucrose kills all bacteria that have the sacB gene.
The now yielded colonies have to be screened for lost antibiotic resistance (to avoid choosing bacteria with a mutation on sacB) and then a colony PCR is performed to confirm the knock-out.
Everything worked until the last step. But no colony had lost the gene...
sahib lopez
New member
re: WC 100K Post Challenge - You Ready?!
o.o that explanation to make a strain is complicated
o.o that explanation to make a strain is complicated
Chregu
New member
re: WC 100K Post Challenge - You Ready?!
It's like with everything. It sound complicated but as soon as you do it on a daily basis it's just everyday work ;-)
It's like with everything. It sound complicated but as soon as you do it on a daily basis it's just everyday work ;-)
Chregu
New member
re: WC 100K Post Challenge - You Ready?!
It's raining heavily, no fun to cycle home. And then I still have to get groceries. Well, it's fine I guess.
It's raining heavily, no fun to cycle home. And then I still have to get groceries. Well, it's fine I guess.
sahib lopez
New member
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