WC 1M Post Challenge - You Ready?!

[MSFTisMIA]

At least if people don't expel me with torches and pitchforks when I don't own a Windows Phone device anymore ;-)

Why would we do that when we knew about your closet love for iOS since last year?

 

[MSFTisMIA]

re: WC 100K Post Challenge - You Ready?!

We have Sahib who uses an iPod. We have MIA who uses Android. I use both WP and Android. There are others using iPhones and Android devices. I don't think we'll get out the burning pitch and tie you to a steak just yet.

I wouldn't tie him to a steak because I don't want to disrespect the meat, but to a floating piece of wood may not be a bad idea......

 

[N_LaRUE]

re: WC 100K Post Challenge - You Ready?!

I wouldn't tie him to a steak because I don't want to disrespect the meat, but to a floating piece of wood may not be a bad idea...��...

LOL! Well a little charged Chregu never hurt a steak I'm sure.

 

[sahib lopez]

re: WC 100K Post Challenge - You Ready?!

Relax, go there without too many expectations. People change in 4 years, just try to have a good time and be open for everything

yeah yeah but still can't blame me for being excited


Sent from my iPod touch using Tapatalk

 

[sahib lopez]

WPC 75K Post Challenge - You Ready?!

LOL! Well a little charged Chregu never hurt a steak I'm sure.

Burn the infidel! *hides my iPod *


Sent from my iPod touch using Tapatalk

 

[MSFTisMIA]

re: WC 100K Post Challenge - You Ready?!

You know, I was really thinking about getting Nexus 5 and this MSFT Android talk may spur those efforts. I'm honestly so so about it, but I may try to go Google lite if I do so - no Gmail and some other things. I remember those pure AOSP/Gapp free ROM days...but that **** is done now...

 

[sahib lopez]

re: WC 100K Post Challenge - You Ready?!

You know, I was really thinking about getting Nexus 5 and this MSFT Android talk may spur those efforts. I'm honestly so so about it, but I may try to go Google lite if I do so - no Gmail and some other things. I remember those pure AOSP/Gapp free ROM days...but that **** is done now...

Sad to see msft is thinking about an android device . No matter how much people say it's the way to go, it's not my go to device . I use my devices as an extension to my tablet not the other they way around. Msft these days baffles me with the choices they are making


Sent from my iPod touch using Tapatalk

 

[sd173]

re: WC 100K Post Challenge - You Ready?!

Is it not possible to play a video through an SD card on WP 8.1? That's what it seems like and that would be really dumb if it's true.

 

[N_LaRUE]

re: WC 100K Post Challenge - You Ready?!

Is it not possible to play a video through an SD card on WP 8.1? That's what it seems like and that would be really dumb if it's true.

Have you tried a different video? I was thinking that maybe the other video had DRM or something. But then it wouldn't surprise me if MS didn't allow video playback off SD card but then I haven't seen complaints about it either.

 

[sd173]

re: WC 100K Post Challenge - You Ready?!

Have you tried a different video? I was thinking that maybe the other video had DRM or something. But then it wouldn't surprise me if MS didn't allow video playback off SD card but then I haven't seen complaints about it either.

I only have one Xbox video file but I'm thinking it has to be something with the DRM since the Windows 8.1 file explorer dialogue box said that the file would lose some permissions if I moved it to the SD card.

 

[SlingLaker9811]

re: WC 100K Post Challenge - You Ready?!

My experiment was not successful. All the strains don't have the deletion I wanted to introduce. Man, that's ****ed up.

What was the experiment about? : Curious:

 

[sd173]

re: WC 100K Post Challenge - You Ready?!

The worst part is I can't move the same video that I downloaded through the WP 8.1 app since it's stuck in the app and not visible though the Files app.

 

[Chregu]

re: WC 100K Post Challenge - You Ready?!

What was the experiment about? : Curious:

I'm trying to create a bacteria strain that is very important for my experiments. It's already pretty sick and I need to knock-out an additional gene. It didn't work. It was the second time I tried...

 

[Chregu]

re: WC 100K Post Challenge - You Ready?!

I have to try out the LG G3 in a store. I think it's an amazing phone that is much cheaper than the S5 or M8. But unfortunately it's also huge.

 

[SlingLaker9811]

re: WC 100K Post Challenge - You Ready?!

How do you create it(the strain)? Do you have to insert the desired gene into a vector and then it binds with the plasmid of the bacteria to produce the desired effect?

 

[Chregu]

re: WC 100K Post Challenge - You Ready?!

How do you create it(the strain)? Do you have to insert the desired gene into a vector and then it binds with the plasmid of the bacteria to produce the desired effect?

It's a double recombination with a suicide vector. To knock-out the gene first around 600 bp of the DNA upstream and downstream of the gene is cloned into a vector. After bringing the vector into the bacteria, these two regions then recombine with a very low efficiency and remove the gene while bringing in a antibiotic resistance cassette and a sacB, a special gene that is important later. By selection for the antibiotic resistance colonies with the recombination can by gained. These will then be grown in a liquid culture again to make a second recombination, losing the resistance cassette and sacB. You then do a second selection by growing the bacteria on sucrose. Sucrose kills all bacteria that have the sacB gene.

The now yielded colonies have to be screened for lost antibiotic resistance (to avoid choosing bacteria with a mutation on sacB) and then a colony PCR is performed to confirm the knock-out.

Everything worked until the last step. But no colony had lost the gene...

 

[sahib lopez]

re: WC 100K Post Challenge - You Ready?!

o.o that explanation to make a strain is complicated

 

[Chregu]

re: WC 100K Post Challenge - You Ready?!

o.o that explanation to make a strain is complicated

It's like with everything. It sound complicated but as soon as you do it on a daily basis it's just everyday work ;-)

 

[Chregu]

re: WC 100K Post Challenge - You Ready?!

It's raining heavily, no fun to cycle home. And then I still have to get groceries. Well, it's fine I guess.

 

[sahib lopez]

re: WC 100K Post Challenge - You Ready?!

It's like with everything. It sound complicated but as soon as you do it on a daily basis it's just everyday work ;-)

yeah let me get edumacated on micro organisms